Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_24_S14_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_24_S14_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8756
Pairs without alignments under any condition 25507
Pairs that did not map uniquely 5737
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 226614
Methylated C's in CpG context 1917
Methylated C's in CHG context 915
Methylated C's in CHH context 32202
Methylated C's in Unknown context 345
Unmethylated C's in CpG context 25011
Unmethylated C's in CHG context 34777
Unmethylated C's in CHH context 131792
Unmethylated C's in Unknown context 748
Percentage methylation (CpG context) 7.1%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 19.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4329 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4427 original bottom strand

Deduplication

Alignments analysed 8756
Unique alignments 7143
Duplicates removed 1613
Duplicated alignments were found at 1199 different positions

Cytosine Methylation after Extraction

Total C's analysed 116054
Methylated C's in CpG context 964
Methylated C's in CHG context 470
Methylated C's in CHH context 16364
Unmethylated C's in CpG context 12213
Unmethylated C's in CHG context 16944
Unmethylated C's in CHH context 69099
Percentage methylation (CpG context) 7.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot