Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_11_S13_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_11_S13_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7493
Pairs without alignments under any condition 28174
Pairs that did not map uniquely 4333
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 198465
Methylated C's in CpG context 2053
Methylated C's in CHG context 1047
Methylated C's in CHH context 29952
Methylated C's in Unknown context 367
Unmethylated C's in CpG context 22952
Unmethylated C's in CHG context 31718
Unmethylated C's in CHH context 110743
Unmethylated C's in Unknown context 662
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 21.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3766 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3727 original bottom strand

Deduplication

Alignments analysed 7493
Unique alignments 5913
Duplicates removed 1580
Duplicated alignments were found at 1144 different positions

Cytosine Methylation after Extraction

Total C's analysed 101581
Methylated C's in CpG context 1015
Methylated C's in CHG context 527
Methylated C's in CHH context 15506
Unmethylated C's in CpG context 11385
Unmethylated C's in CHG context 16086
Unmethylated C's in CHH context 57062
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot