Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_06_S12_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_06_S12_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10796
Pairs without alignments under any condition 24650
Pairs that did not map uniquely 4554
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 311226
Methylated C's in CpG context 32278
Methylated C's in CHG context 1760
Methylated C's in CHH context 35296
Methylated C's in Unknown context 446
Unmethylated C's in CpG context 17065
Unmethylated C's in CHG context 58888
Unmethylated C's in CHH context 165939
Unmethylated C's in Unknown context 729
Percentage methylation (CpG context) 65.4%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 17.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5434 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5362 original bottom strand

Deduplication

Alignments analysed 10796
Unique alignments 8593
Duplicates removed 2203
Duplicated alignments were found at 1623 different positions

Cytosine Methylation after Extraction

Total C's analysed 152888
Methylated C's in CpG context 15568
Methylated C's in CHG context 893
Methylated C's in CHH context 17790
Unmethylated C's in CpG context 8335
Unmethylated C's in CHG context 28551
Unmethylated C's in CHH context 81751
Percentage methylation (CpG context) 65.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot