Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_24_S11_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_24_S11_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 12012
Pairs without alignments under any condition 21970
Pairs that did not map uniquely 6018
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 323750
Methylated C's in CpG context 37380
Methylated C's in CHG context 2071
Methylated C's in CHH context 45327
Methylated C's in Unknown context 514
Unmethylated C's in CpG context 14043
Unmethylated C's in CHG context 62328
Unmethylated C's in CHH context 162601
Unmethylated C's in Unknown context 839
Percentage methylation (CpG context) 72.7%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 21.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6056 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5956 original bottom strand

Deduplication

Alignments analysed 12012
Unique alignments 9720
Duplicates removed 2292
Duplicated alignments were found at 1697 different positions

Cytosine Methylation after Extraction

Total C's analysed 151222
Methylated C's in CpG context 16840
Methylated C's in CHG context 975
Methylated C's in CHH context 23385
Unmethylated C's in CpG context 6428
Unmethylated C's in CHG context 28243
Unmethylated C's in CHH context 75351
Percentage methylation (CpG context) 72.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot