Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_21_S10_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_21_S10_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11289
Pairs without alignments under any condition 23680
Pairs that did not map uniquely 5031
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 314760
Methylated C's in CpG context 28906
Methylated C's in CHG context 1995
Methylated C's in CHH context 46988
Methylated C's in Unknown context 583
Unmethylated C's in CpG context 15041
Unmethylated C's in CHG context 59843
Unmethylated C's in CHH context 161987
Unmethylated C's in Unknown context 747
Percentage methylation (CpG context) 65.8%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 22.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5692 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5597 original bottom strand

Deduplication

Alignments analysed 11289
Unique alignments 8671
Duplicates removed 2618
Duplicated alignments were found at 1823 different positions

Cytosine Methylation after Extraction

Total C's analysed 146989
Methylated C's in CpG context 12986
Methylated C's in CHG context 944
Methylated C's in CHH context 23967
Unmethylated C's in CpG context 6947
Unmethylated C's in CHG context 27154
Unmethylated C's in CHH context 74991
Percentage methylation (CpG context) 65.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot