Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_06_S9_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_06_S9_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7376
Pairs without alignments under any condition 27780
Pairs that did not map uniquely 4844
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 204803
Methylated C's in CpG context 1999
Methylated C's in CHG context 803
Methylated C's in CHH context 31268
Methylated C's in Unknown context 295
Unmethylated C's in CpG context 23855
Unmethylated C's in CHG context 31829
Unmethylated C's in CHH context 115049
Unmethylated C's in Unknown context 631
Percentage methylation (CpG context) 7.7%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 21.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3752 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3624 original bottom strand

Deduplication

Alignments analysed 7376
Unique alignments 5929
Duplicates removed 1447
Duplicated alignments were found at 1041 different positions

Cytosine Methylation after Extraction

Total C's analysed 112742
Methylated C's in CpG context 1080
Methylated C's in CHG context 468
Methylated C's in CHH context 17570
Unmethylated C's in CpG context 12749
Unmethylated C's in CHG context 17129
Unmethylated C's in CHH context 63746
Percentage methylation (CpG context) 7.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot