Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_01_S8_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_01_S8_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10115
Pairs without alignments under any condition 22135
Pairs that did not map uniquely 7750
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 252320
Methylated C's in CpG context 15573
Methylated C's in CHG context 1636
Methylated C's in CHH context 67912
Methylated C's in Unknown context 655
Unmethylated C's in CpG context 13980
Unmethylated C's in CHG context 39452
Unmethylated C's in CHH context 113767
Unmethylated C's in Unknown context 756
Percentage methylation (CpG context) 52.7%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 37.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5058 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5057 original bottom strand

Deduplication

Alignments analysed 10115
Unique alignments 8490
Duplicates removed 1625
Duplicated alignments were found at 1255 different positions

Cytosine Methylation after Extraction

Total C's analysed 125596
Methylated C's in CpG context 7293
Methylated C's in CHG context 860
Methylated C's in CHH context 36982
Unmethylated C's in CpG context 6707
Unmethylated C's in CHG context 18644
Unmethylated C's in CHH context 55110
Percentage methylation (CpG context) 52.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot