Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_33_S7_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_33_S7_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10814
Pairs without alignments under any condition 25418
Pairs that did not map uniquely 3768
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 335864
Methylated C's in CpG context 42232
Methylated C's in CHG context 1769
Methylated C's in CHH context 27001
Methylated C's in Unknown context 364
Unmethylated C's in CpG context 15307
Unmethylated C's in CHG context 67871
Unmethylated C's in CHH context 181684
Unmethylated C's in Unknown context 692
Percentage methylation (CpG context) 73.4%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 12.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5390 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5424 original bottom strand

Deduplication

Alignments analysed 10814
Unique alignments 8530
Duplicates removed 2284
Duplicated alignments were found at 1635 different positions

Cytosine Methylation after Extraction

Total C's analysed 165221
Methylated C's in CpG context 20278
Methylated C's in CHG context 892
Methylated C's in CHH context 13515
Unmethylated C's in CpG context 7562
Unmethylated C's in CHG context 33023
Unmethylated C's in CHH context 89951
Percentage methylation (CpG context) 72.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot