Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7899
Pairs without alignments under any condition 26920
Pairs that did not map uniquely 5181
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 202738
Methylated C's in CpG context 5203
Methylated C's in CHG context 1037
Methylated C's in CHH context 36932
Methylated C's in Unknown context 364
Unmethylated C's in CpG context 22277
Unmethylated C's in CHG context 32441
Unmethylated C's in CHH context 104848
Unmethylated C's in Unknown context 581
Percentage methylation (CpG context) 18.9%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 26.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3960 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3939 original bottom strand

Deduplication

Alignments analysed 7899
Unique alignments 6334
Duplicates removed 1565
Duplicated alignments were found at 1153 different positions

Cytosine Methylation after Extraction

Total C's analysed 101586
Methylated C's in CpG context 2571
Methylated C's in CHG context 519
Methylated C's in CHH context 19440
Unmethylated C's in CpG context 10793
Unmethylated C's in CHG context 15806
Unmethylated C's in CHH context 52457
Percentage methylation (CpG context) 19.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot