Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_04_S5_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_04_S5_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8490
Pairs without alignments under any condition 22932
Pairs that did not map uniquely 8578
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 205762
Methylated C's in CpG context 2904
Methylated C's in CHG context 1192
Methylated C's in CHH context 69174
Methylated C's in Unknown context 663
Unmethylated C's in CpG context 20728
Unmethylated C's in CHG context 26444
Unmethylated C's in CHH context 85320
Unmethylated C's in Unknown context 742
Percentage methylation (CpG context) 12.3%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 44.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4274 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4216 original bottom strand

Deduplication

Alignments analysed 8490
Unique alignments 7208
Duplicates removed 1282
Duplicated alignments were found at 977 different positions

Cytosine Methylation after Extraction

Total C's analysed 105791
Methylated C's in CpG context 1431
Methylated C's in CHG context 694
Methylated C's in CHH context 38411
Unmethylated C's in CpG context 10128
Unmethylated C's in CHG context 12668
Unmethylated C's in CHH context 42459
Percentage methylation (CpG context) 12.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot