Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_38_S4_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_38_S4_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11078
Pairs without alignments under any condition 23464
Pairs that did not map uniquely 5458
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 297178
Methylated C's in CpG context 29290
Methylated C's in CHG context 1571
Methylated C's in CHH context 39695
Methylated C's in Unknown context 517
Unmethylated C's in CpG context 13207
Unmethylated C's in CHG context 55011
Unmethylated C's in CHH context 158404
Unmethylated C's in Unknown context 854
Percentage methylation (CpG context) 68.9%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 20.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5463 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5615 original bottom strand

Deduplication

Alignments analysed 11078
Unique alignments 8934
Duplicates removed 2144
Duplicated alignments were found at 1618 different positions

Cytosine Methylation after Extraction

Total C's analysed 138213
Methylated C's in CpG context 13139
Methylated C's in CHG context 789
Methylated C's in CHH context 20754
Unmethylated C's in CpG context 6233
Unmethylated C's in CHG context 24844
Unmethylated C's in CHH context 72454
Percentage methylation (CpG context) 67.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot