Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_22_S3_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_22_S3_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11356
Pairs without alignments under any condition 23683
Pairs that did not map uniquely 4961
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 333983
Methylated C's in CpG context 34384
Methylated C's in CHG context 1468
Methylated C's in CHH context 30589
Methylated C's in Unknown context 380
Unmethylated C's in CpG context 16321
Unmethylated C's in CHG context 64313
Unmethylated C's in CHH context 186908
Unmethylated C's in Unknown context 799
Percentage methylation (CpG context) 67.8%
Percentage methylation (CHG context) 2.2%
Percentage methylation (CHH context) 14.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5637 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5719 original bottom strand

Deduplication

Alignments analysed 11356
Unique alignments 9169
Duplicates removed 2187
Duplicated alignments were found at 1654 different positions

Cytosine Methylation after Extraction

Total C's analysed 162827
Methylated C's in CpG context 16540
Methylated C's in CHG context 723
Methylated C's in CHH context 15401
Unmethylated C's in CpG context 7983
Unmethylated C's in CHG context 30923
Unmethylated C's in CHH context 91257
Percentage methylation (CpG context) 67.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot