Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_14_S2_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_14_S2_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11031
Pairs without alignments under any condition 23040
Pairs that did not map uniquely 5929
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 294198
Methylated C's in CpG context 30190
Methylated C's in CHG context 1500
Methylated C's in CHH context 35786
Methylated C's in Unknown context 427
Unmethylated C's in CpG context 14240
Unmethylated C's in CHG context 53223
Unmethylated C's in CHH context 159259
Unmethylated C's in Unknown context 902
Percentage methylation (CpG context) 67.9%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 18.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5408 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5623 original bottom strand

Deduplication

Alignments analysed 11031
Unique alignments 8719
Duplicates removed 2312
Duplicated alignments were found at 1629 different positions

Cytosine Methylation after Extraction

Total C's analysed 131560
Methylated C's in CpG context 12936
Methylated C's in CHG context 710
Methylated C's in CHH context 17530
Unmethylated C's in CpG context 6303
Unmethylated C's in CHG context 23241
Unmethylated C's in CHH context 70840
Percentage methylation (CpG context) 67.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot