Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_06_S1_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_06_S1_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11258
Pairs without alignments under any condition 24154
Pairs that did not map uniquely 4588
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 335869
Methylated C's in CpG context 42201
Methylated C's in CHG context 1808
Methylated C's in CHH context 28990
Methylated C's in Unknown context 320
Unmethylated C's in CpG context 14292
Unmethylated C's in CHG context 66833
Unmethylated C's in CHH context 181745
Unmethylated C's in Unknown context 751
Percentage methylation (CpG context) 74.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 13.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5622 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5636 original bottom strand

Deduplication

Alignments analysed 11258
Unique alignments 9085
Duplicates removed 2173
Duplicated alignments were found at 1632 different positions

Cytosine Methylation after Extraction

Total C's analysed 162164
Methylated C's in CpG context 20131
Methylated C's in CHG context 823
Methylated C's in CHH context 14365
Unmethylated C's in CpG context 6928
Unmethylated C's in CHG context 32018
Unmethylated C's in CHH context 87899
Percentage methylation (CpG context) 74.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot