Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_11_S20_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_11_S20_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11468
Pairs without alignments under any condition 22715
Pairs that did not map uniquely 5817
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 303522
Methylated C's in CpG context 28865
Methylated C's in CHG context 1795
Methylated C's in CHH context 38188
Methylated C's in Unknown context 441
Unmethylated C's in CpG context 13798
Unmethylated C's in CHG context 55713
Unmethylated C's in CHH context 165163
Unmethylated C's in Unknown context 912
Percentage methylation (CpG context) 67.7%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 18.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5702 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5766 original bottom strand

Deduplication

Alignments analysed 11468
Unique alignments 9261
Duplicates removed 2207
Duplicated alignments were found at 1678 different positions

Cytosine Methylation after Extraction

Total C's analysed 139697
Methylated C's in CpG context 12950
Methylated C's in CHG context 853
Methylated C's in CHH context 19246
Unmethylated C's in CpG context 6215
Unmethylated C's in CHG context 25122
Unmethylated C's in CHH context 75311
Percentage methylation (CpG context) 67.6%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot