Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_08_S19_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_08_S19_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 9907
Pairs without alignments under any condition 22552
Pairs that did not map uniquely 7541
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 246661
Methylated C's in CpG context 9354
Methylated C's in CHG context 1670
Methylated C's in CHH context 69624
Methylated C's in Unknown context 697
Unmethylated C's in CpG context 18049
Unmethylated C's in CHG context 36621
Unmethylated C's in CHH context 111343
Unmethylated C's in Unknown context 842
Percentage methylation (CpG context) 34.1%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 38.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4914 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4993 original bottom strand

Deduplication

Alignments analysed 9907
Unique alignments 8269
Duplicates removed 1638
Duplicated alignments were found at 1219 different positions

Cytosine Methylation after Extraction

Total C's analysed 125903
Methylated C's in CpG context 4529
Methylated C's in CHG context 861
Methylated C's in CHH context 38101
Unmethylated C's in CpG context 8891
Unmethylated C's in CHG context 18073
Unmethylated C's in CHH context 55448
Percentage methylation (CpG context) 33.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot