Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_01_S18_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_01_S18_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11719
Pairs without alignments under any condition 23157
Pairs that did not map uniquely 5124
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 326695
Methylated C's in CpG context 35976
Methylated C's in CHG context 2121
Methylated C's in CHH context 33948
Methylated C's in Unknown context 457
Unmethylated C's in CpG context 13425
Unmethylated C's in CHG context 63208
Unmethylated C's in CHH context 178017
Unmethylated C's in Unknown context 725
Percentage methylation (CpG context) 72.8%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 16.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5784 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5935 original bottom strand

Deduplication

Alignments analysed 11719
Unique alignments 9676
Duplicates removed 2043
Duplicated alignments were found at 1570 different positions

Cytosine Methylation after Extraction

Total C's analysed 161633
Methylated C's in CpG context 17616
Methylated C's in CHG context 1021
Methylated C's in CHH context 16826
Unmethylated C's in CpG context 6537
Unmethylated C's in CHG context 31004
Unmethylated C's in CHH context 88629
Percentage methylation (CpG context) 72.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot