Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_28_S17_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_28_S17_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10820
Pairs without alignments under any condition 24913
Pairs that did not map uniquely 4267
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 306228
Methylated C's in CpG context 33551
Methylated C's in CHG context 2155
Methylated C's in CHH context 31153
Methylated C's in Unknown context 396
Unmethylated C's in CpG context 13846
Unmethylated C's in CHG context 59723
Unmethylated C's in CHH context 165800
Unmethylated C's in Unknown context 771
Percentage methylation (CpG context) 70.8%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 15.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5404 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5416 original bottom strand

Deduplication

Alignments analysed 10820
Unique alignments 8665
Duplicates removed 2155
Duplicated alignments were found at 1626 different positions

Cytosine Methylation after Extraction

Total C's analysed 150440
Methylated C's in CpG context 15884
Methylated C's in CHG context 1059
Methylated C's in CHH context 15581
Unmethylated C's in CpG context 6733
Unmethylated C's in CHG context 29149
Unmethylated C's in CHH context 82034
Percentage methylation (CpG context) 70.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot