Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_13_S16_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_13_S16_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 12161
Pairs without alignments under any condition 21599
Pairs that did not map uniquely 6240
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 357895
Methylated C's in CpG context 21379
Methylated C's in CHG context 1799
Methylated C's in CHH context 48791
Methylated C's in Unknown context 508
Unmethylated C's in CpG context 19792
Unmethylated C's in CHG context 59742
Unmethylated C's in CHH context 206392
Unmethylated C's in Unknown context 818
Percentage methylation (CpG context) 51.9%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 19.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6138 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6023 original bottom strand

Deduplication

Alignments analysed 12161
Unique alignments 9873
Duplicates removed 2288
Duplicated alignments were found at 1699 different positions

Cytosine Methylation after Extraction

Total C's analysed 183761
Methylated C's in CpG context 10389
Methylated C's in CHG context 919
Methylated C's in CHH context 25891
Unmethylated C's in CpG context 9990
Unmethylated C's in CHG context 29628
Unmethylated C's in CHH context 106944
Percentage methylation (CpG context) 51.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot