Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_02_S15_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_02_S15_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11594
Pairs without alignments under any condition 21966
Pairs that did not map uniquely 6440
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 298641
Methylated C's in CpG context 30191
Methylated C's in CHG context 2027
Methylated C's in CHH context 46592
Methylated C's in Unknown context 567
Unmethylated C's in CpG context 13565
Unmethylated C's in CHG context 53212
Unmethylated C's in CHH context 153054
Unmethylated C's in Unknown context 881
Percentage methylation (CpG context) 69.0%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 23.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5847 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5747 original bottom strand

Deduplication

Alignments analysed 11594
Unique alignments 9368
Duplicates removed 2226
Duplicated alignments were found at 1649 different positions

Cytosine Methylation after Extraction

Total C's analysed 137756
Methylated C's in CpG context 13275
Methylated C's in CHG context 960
Methylated C's in CHH context 24621
Unmethylated C's in CpG context 6166
Unmethylated C's in CHG context 23287
Unmethylated C's in CHH context 69447
Percentage methylation (CpG context) 68.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot