Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_24_S14_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_24_S14_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8617
Pairs without alignments under any condition 24320
Pairs that did not map uniquely 7063
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 220377
Methylated C's in CpG context 2042
Methylated C's in CHG context 937
Methylated C's in CHH context 33312
Methylated C's in Unknown context 359
Unmethylated C's in CpG context 23000
Unmethylated C's in CHG context 33654
Unmethylated C's in CHH context 127432
Unmethylated C's in Unknown context 696
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 20.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4224 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4393 original bottom strand

Deduplication

Alignments analysed 8617
Unique alignments 7019
Duplicates removed 1598
Duplicated alignments were found at 1169 different positions

Cytosine Methylation after Extraction

Total C's analysed 113556
Methylated C's in CpG context 1029
Methylated C's in CHG context 490
Methylated C's in CHH context 17040
Unmethylated C's in CpG context 11246
Unmethylated C's in CHG context 16555
Unmethylated C's in CHH context 67196
Percentage methylation (CpG context) 8.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot