Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_11_S13_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_11_S13_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7401
Pairs without alignments under any condition 27194
Pairs that did not map uniquely 5405
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 194680
Methylated C's in CpG context 2160
Methylated C's in CHG context 1042
Methylated C's in CHH context 30653
Methylated C's in Unknown context 384
Unmethylated C's in CpG context 21368
Unmethylated C's in CHG context 30637
Unmethylated C's in CHH context 108820
Unmethylated C's in Unknown context 642
Percentage methylation (CpG context) 9.2%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 22.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3709 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3692 original bottom strand

Deduplication

Alignments analysed 7401
Unique alignments 5827
Duplicates removed 1574
Duplicated alignments were found at 1140 different positions

Cytosine Methylation after Extraction

Total C's analysed 100121
Methylated C's in CpG context 1079
Methylated C's in CHG context 522
Methylated C's in CHH context 16038
Unmethylated C's in CpG context 10663
Unmethylated C's in CHG context 15566
Unmethylated C's in CHH context 56253
Percentage methylation (CpG context) 9.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot