Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_06_S12_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH07_06_S12_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11241
Pairs without alignments under any condition 23687
Pairs that did not map uniquely 5072
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 324742
Methylated C's in CpG context 34758
Methylated C's in CHG context 1840
Methylated C's in CHH context 36921
Methylated C's in Unknown context 456
Unmethylated C's in CpG context 16926
Unmethylated C's in CHG context 61450
Unmethylated C's in CHH context 172847
Unmethylated C's in Unknown context 773
Percentage methylation (CpG context) 67.3%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 17.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5682 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5559 original bottom strand

Deduplication

Alignments analysed 11241
Unique alignments 8923
Duplicates removed 2318
Duplicated alignments were found at 1716 different positions

Cytosine Methylation after Extraction

Total C's analysed 159318
Methylated C's in CpG context 16744
Methylated C's in CHG context 912
Methylated C's in CHH context 18743
Unmethylated C's in CpG context 8300
Unmethylated C's in CHG context 29745
Unmethylated C's in CHH context 84874
Percentage methylation (CpG context) 66.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot