Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_24_S11_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_24_S11_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 12588
Pairs without alignments under any condition 21090
Pairs that did not map uniquely 6322
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 340319
Methylated C's in CpG context 40260
Methylated C's in CHG context 2150
Methylated C's in CHH context 46396
Methylated C's in Unknown context 483
Unmethylated C's in CpG context 14564
Unmethylated C's in CHG context 66283
Unmethylated C's in CHH context 170666
Unmethylated C's in Unknown context 845
Percentage methylation (CpG context) 73.4%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 21.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6364 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6224 original bottom strand

Deduplication

Alignments analysed 12588
Unique alignments 10151
Duplicates removed 2437
Duplicated alignments were found at 1798 different positions

Cytosine Methylation after Extraction

Total C's analysed 157879
Methylated C's in CpG context 18130
Methylated C's in CHG context 1029
Methylated C's in CHH context 23490
Unmethylated C's in CpG context 6681
Unmethylated C's in CHG context 30022
Unmethylated C's in CHH context 78527
Percentage methylation (CpG context) 73.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot