Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_21_S10_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_21_S10_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11871
Pairs without alignments under any condition 22766
Pairs that did not map uniquely 5363
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 332448
Methylated C's in CpG context 31036
Methylated C's in CHG context 2093
Methylated C's in CHH context 49972
Methylated C's in Unknown context 561
Unmethylated C's in CpG context 15260
Unmethylated C's in CHG context 63202
Unmethylated C's in CHH context 170885
Unmethylated C's in Unknown context 732
Percentage methylation (CpG context) 67.0%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 22.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5975 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5896 original bottom strand

Deduplication

Alignments analysed 11871
Unique alignments 9140
Duplicates removed 2731
Duplicated alignments were found at 1895 different positions

Cytosine Methylation after Extraction

Total C's analysed 155759
Methylated C's in CpG context 13950
Methylated C's in CHG context 1012
Methylated C's in CHH context 25631
Unmethylated C's in CpG context 7182
Unmethylated C's in CHG context 28767
Unmethylated C's in CHH context 79217
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot