Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_06_S9_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_06_S9_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7392
Pairs without alignments under any condition 26796
Pairs that did not map uniquely 5812
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 200086
Methylated C's in CpG context 2119
Methylated C's in CHG context 815
Methylated C's in CHH context 31548
Methylated C's in Unknown context 260
Unmethylated C's in CpG context 22432
Unmethylated C's in CHG context 31203
Unmethylated C's in CHH context 111969
Unmethylated C's in Unknown context 533
Percentage methylation (CpG context) 8.6%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 22.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3734 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3658 original bottom strand

Deduplication

Alignments analysed 7392
Unique alignments 5906
Duplicates removed 1486
Duplicated alignments were found at 1075 different positions

Cytosine Methylation after Extraction

Total C's analysed 109463
Methylated C's in CpG context 1150
Methylated C's in CHG context 478
Methylated C's in CHH context 17607
Unmethylated C's in CpG context 11937
Unmethylated C's in CHG context 16737
Unmethylated C's in CHH context 61554
Percentage methylation (CpG context) 8.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot