Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_01_S8_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH05_01_S8_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10452
Pairs without alignments under any condition 21322
Pairs that did not map uniquely 8226
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 261122
Methylated C's in CpG context 16430
Methylated C's in CHG context 1755
Methylated C's in CHH context 70453
Methylated C's in Unknown context 682
Unmethylated C's in CpG context 14047
Unmethylated C's in CHG context 40834
Unmethylated C's in CHH context 117603
Unmethylated C's in Unknown context 769
Percentage methylation (CpG context) 53.9%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 37.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5252 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5200 original bottom strand

Deduplication

Alignments analysed 10452
Unique alignments 8765
Duplicates removed 1687
Duplicated alignments were found at 1304 different positions

Cytosine Methylation after Extraction

Total C's analysed 129943
Methylated C's in CpG context 7713
Methylated C's in CHG context 896
Methylated C's in CHH context 38267
Unmethylated C's in CpG context 6771
Unmethylated C's in CHG context 19227
Unmethylated C's in CHH context 57069
Percentage methylation (CpG context) 53.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot