Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_33_S7_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_33_S7_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11375
Pairs without alignments under any condition 24481
Pairs that did not map uniquely 4144
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 353110
Methylated C's in CpG context 45645
Methylated C's in CHG context 1877
Methylated C's in CHH context 26628
Methylated C's in Unknown context 373
Unmethylated C's in CpG context 15552
Unmethylated C's in CHG context 72198
Unmethylated C's in CHH context 191210
Unmethylated C's in Unknown context 650
Percentage methylation (CpG context) 74.6%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 12.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5675 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5700 original bottom strand

Deduplication

Alignments analysed 11375
Unique alignments 8973
Duplicates removed 2402
Duplicated alignments were found at 1720 different positions

Cytosine Methylation after Extraction

Total C's analysed 174348
Methylated C's in CpG context 22001
Methylated C's in CHG context 941
Methylated C's in CHH context 13365
Unmethylated C's in CpG context 7701
Unmethylated C's in CHG context 35283
Unmethylated C's in CHH context 95057
Percentage methylation (CpG context) 74.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot