Bismark report for: /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/dumoar/sc-con-genome/ with the specified options: -q --score-min L,0,-0.6 -p 40 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 40000 Number of paired-end alignments with a unique best hit: 7978 Mapping efficiency: 19.9% Sequence pairs with no alignments under any condition: 25966 Sequence pairs did not map uniquely: 6056 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3975 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 4003 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 203797 Total methylated C's in CpG context: 5548 Total methylated C's in CHG context: 1087 Total methylated C's in CHH context: 39079 Total methylated C's in Unknown context: 348 Total unmethylated C's in CpG context: 21216 Total unmethylated C's in CHG context: 32439 Total unmethylated C's in CHH context: 104428 Total unmethylated C's in Unknown context: 605 C methylated in CpG context: 20.7% C methylated in CHG context: 3.2% C methylated in CHH context: 27.2% C methylated in unknown context (CN or CHN): 36.5% Bismark completed in 0d 0h 0m 56s