Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_15_S6_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7978
Pairs without alignments under any condition 25966
Pairs that did not map uniquely 6056
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 203797
Methylated C's in CpG context 5548
Methylated C's in CHG context 1087
Methylated C's in CHH context 39079
Methylated C's in Unknown context 348
Unmethylated C's in CpG context 21216
Unmethylated C's in CHG context 32439
Unmethylated C's in CHH context 104428
Unmethylated C's in Unknown context 605
Percentage methylation (CpG context) 20.7%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 27.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3975 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4003 original bottom strand

Deduplication

Alignments analysed 7978
Unique alignments 6405
Duplicates removed 1573
Duplicated alignments were found at 1152 different positions

Cytosine Methylation after Extraction

Total C's analysed 102885
Methylated C's in CpG context 2746
Methylated C's in CHG context 547
Methylated C's in CHH context 20652
Unmethylated C's in CpG context 10417
Unmethylated C's in CHG context 15940
Unmethylated C's in CHH context 52583
Percentage methylation (CpG context) 20.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot