Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_04_S5_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH03_04_S5_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8475
Pairs without alignments under any condition 22031
Pairs that did not map uniquely 9494
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 200080
Methylated C's in CpG context 3062
Methylated C's in CHG context 1268
Methylated C's in CHH context 67834
Methylated C's in Unknown context 659
Unmethylated C's in CpG context 19389
Unmethylated C's in CHG context 25947
Unmethylated C's in CHH context 82580
Unmethylated C's in Unknown context 705
Percentage methylation (CpG context) 13.6%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 45.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4235 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4240 original bottom strand

Deduplication

Alignments analysed 8475
Unique alignments 7228
Duplicates removed 1247
Duplicated alignments were found at 944 different positions

Cytosine Methylation after Extraction

Total C's analysed 103908
Methylated C's in CpG context 1499
Methylated C's in CHG context 738
Methylated C's in CHH context 38465
Unmethylated C's in CpG context 9573
Unmethylated C's in CHG context 12513
Unmethylated C's in CHH context 41120
Percentage methylation (CpG context) 13.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot