Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_38_S4_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_38_S4_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11572
Pairs without alignments under any condition 22692
Pairs that did not map uniquely 5736
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 309729
Methylated C's in CpG context 31379
Methylated C's in CHG context 1708
Methylated C's in CHH context 39483
Methylated C's in Unknown context 486
Unmethylated C's in CpG context 13283
Unmethylated C's in CHG context 57744
Unmethylated C's in CHH context 166132
Unmethylated C's in Unknown context 868
Percentage methylation (CpG context) 70.3%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 19.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5727 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5845 original bottom strand

Deduplication

Alignments analysed 11572
Unique alignments 9332
Duplicates removed 2240
Duplicated alignments were found at 1699 different positions

Cytosine Methylation after Extraction

Total C's analysed 144393
Methylated C's in CpG context 14129
Methylated C's in CHG context 865
Methylated C's in CHH context 20560
Unmethylated C's in CpG context 6281
Unmethylated C's in CHG context 26198
Unmethylated C's in CHH context 76360
Percentage methylation (CpG context) 69.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot