Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_22_S3_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_22_S3_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11862
Pairs without alignments under any condition 22771
Pairs that did not map uniquely 5367
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 350298
Methylated C's in CpG context 36949
Methylated C's in CHG context 1595
Methylated C's in CHH context 32057
Methylated C's in Unknown context 383
Unmethylated C's in CpG context 16470
Unmethylated C's in CHG context 67571
Unmethylated C's in CHH context 195656
Unmethylated C's in Unknown context 832
Percentage methylation (CpG context) 69.2%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 14.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5893 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5969 original bottom strand

Deduplication

Alignments analysed 11862
Unique alignments 9573
Duplicates removed 2289
Duplicated alignments were found at 1728 different positions

Cytosine Methylation after Extraction

Total C's analysed 171369
Methylated C's in CpG context 17817
Methylated C's in CHG context 802
Methylated C's in CHH context 16011
Unmethylated C's in CpG context 8137
Unmethylated C's in CHG context 32706
Unmethylated C's in CHH context 95896
Percentage methylation (CpG context) 68.6%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot