Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_14_S2_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_14_S2_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11548
Pairs without alignments under any condition 22152
Pairs that did not map uniquely 6300
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 306759
Methylated C's in CpG context 32725
Methylated C's in CHG context 1594
Methylated C's in CHH context 35464
Methylated C's in Unknown context 457
Unmethylated C's in CpG context 14243
Unmethylated C's in CHG context 56303
Unmethylated C's in CHH context 166430
Unmethylated C's in Unknown context 908
Percentage methylation (CpG context) 69.7%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 17.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5688 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5860 original bottom strand

Deduplication

Alignments analysed 11548
Unique alignments 9102
Duplicates removed 2446
Duplicated alignments were found at 1736 different positions

Cytosine Methylation after Extraction

Total C's analysed 136522
Methylated C's in CpG context 13983
Methylated C's in CHG context 761
Methylated C's in CHH context 17512
Unmethylated C's in CpG context 6349
Unmethylated C's in CHG context 24543
Unmethylated C's in CHH context 73374
Percentage methylation (CpG context) 68.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot