Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_06_S1_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH01_06_S1_L001_R2_001_val_2.fq.gz

Data processed at 13:43 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11805
Pairs without alignments under any condition 23284
Pairs that did not map uniquely 4911
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 353081
Methylated C's in CpG context 45441
Methylated C's in CHG context 1887
Methylated C's in CHH context 29260
Methylated C's in Unknown context 304
Unmethylated C's in CpG context 14595
Unmethylated C's in CHG context 70652
Unmethylated C's in CHH context 191246
Unmethylated C's in Unknown context 718
Percentage methylation (CpG context) 75.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 13.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5871 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5934 original bottom strand

Deduplication

Alignments analysed 11805
Unique alignments 9520
Duplicates removed 2285
Duplicated alignments were found at 1723 different positions

Cytosine Methylation after Extraction

Total C's analysed 170858
Methylated C's in CpG context 21652
Methylated C's in CHG context 860
Methylated C's in CHH context 14743
Unmethylated C's in CpG context 7081
Unmethylated C's in CHG context 33856
Unmethylated C's in CHH context 92666
Percentage methylation (CpG context) 75.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot