Bismark report for: /gscratch/srlab/sr320/data/cg/zr3534_8_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_8_R2.fastp-trim.20201202.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/Cgig-genome/roslin_M/ with the specified options: -q --score-min L,0,-0.6 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 90605411 Number of paired-end alignments with a unique best hit: 55543581 Mapping efficiency: 61.3% Sequence pairs with no alignments under any condition: 20159676 Sequence pairs did not map uniquely: 14902154 Sequence pairs which were discarded because genomic sequence could not be extracted: 132 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 13773128 ((converted) top strand) GA/CT/CT: 13966090 (complementary to (converted) top strand) GA/CT/GA: 13998198 (complementary to (converted) bottom strand) CT/GA/GA: 13806033 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1919611382 Total methylated C's in CpG context: 25390230 Total methylated C's in CHG context: 1605629 Total methylated C's in CHH context: 6877825 Total methylated C's in Unknown context: 64253 Total unmethylated C's in CpG context: 221478350 Total unmethylated C's in CHG context: 332078494 Total unmethylated C's in CHH context: 1332180854 Total unmethylated C's in Unknown context: 4697286 C methylated in CpG context: 10.3% C methylated in CHG context: 0.5% C methylated in CHH context: 0.5% C methylated in unknown context (CN or CHN): 1.3% Bismark completed in 0d 18h 48m 29s