Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg/zr3534_2_R1.fastp-trim.20201202.fq.gz and /gscratch/srlab/sr320/data/cg/zr3534_2_R2.fastp-trim.20201202.fq.gz

Data processed at 23:58 on 2021-03-12


Alignment Stats

Sequence pairs analysed in total 75413899
Paired-end alignments with a unique best hit 46140925
Pairs without alignments under any condition 17015547
Pairs that did not map uniquely 12257427
Genomic sequence context not extractable (edges of chromosomes) 102
61.2%22.6%16.3%0.000135%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 1612045578
Methylated C's in CpG context 21945879
Methylated C's in CHG context 1370392
Methylated C's in CHH context 5818112
Methylated C's in Unknown context 53662
Unmethylated C's in CpG context 190060088
Unmethylated C's in CHG context 281860222
Unmethylated C's in CHH context 1110990885
Unmethylated C's in Unknown context 3919471
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 11482323 original top strand
CTOT 11541123 complementary to original top strand
CTOB 11582832 complementary to original bottom strand
OB 11534545 original bottom strand
OTCTOTCTOBOB02M4M6M8M10M12M
Number of Alignments

Deduplication

Alignments analysed 46140823
Unique alignments 37693953
Duplicates removed 8446870
Duplicated alignments were found at 7047577 different positions
81.7%18.3%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 911253744
Methylated C's in CpG context 12014863
Methylated C's in CHG context 763389
Methylated C's in CHH context 3276821
Unmethylated C's in CpG context 105812698
Unmethylated C's in CHG context 157435632
Unmethylated C's in CHH context 631950341
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

204060801000204060801000.5M1M1.5M2M2.5M3M3.5M4M4.5M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls

2040608010002040608010000.5M1M1.5M2M2.5M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 2Position in Read [bp]% Methylation# Methylation Calls