Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth12.fastp-trim.202003060645_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 217 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 9999773 Sequence pairs did not map uniquely: 10 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 43 ((converted) top strand) GA/CT/CT: 58 (complementary to (converted) top strand) GA/CT/GA: 60 (complementary to (converted) bottom strand) CT/GA/GA: 56 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 10217 Total methylated C's in CpG context: 458 Total methylated C's in CHG context: 574 Total methylated C's in CHH context: 767 Total methylated C's in Unknown context: 8 Total unmethylated C's in CpG context: 2118 Total unmethylated C's in CHG context: 2450 Total unmethylated C's in CHH context: 3850 Total unmethylated C's in Unknown context: 27 C methylated in CpG context: 17.8% C methylated in CHG context: 19.0% C methylated in CHH context: 16.6% C methylated in unknown context (CN or CHN): 22.9% Bismark completed in 0d 0h 6m 11s