Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth1.fastp-trim.202003061245_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth1.fastp-trim.202003061245_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/lambda/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 444 Mapping efficiency: 0.0% Sequence pairs with no alignments under any condition: 9999522 Sequence pairs did not map uniquely: 34 Sequence pairs which were discarded because genomic sequence could not be extracted: 2 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 103 ((converted) top strand) GA/CT/CT: 127 (complementary to (converted) top strand) GA/CT/GA: 101 (complementary to (converted) bottom strand) CT/GA/GA: 111 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 17823 Total methylated C's in CpG context: 117 Total methylated C's in CHG context: 270 Total methylated C's in CHH context: 396 Total methylated C's in Unknown context: 15 Total unmethylated C's in CpG context: 4345 Total unmethylated C's in CHG context: 4832 Total unmethylated C's in CHH context: 7863 Total unmethylated C's in Unknown context: 60 C methylated in CpG context: 2.6% C methylated in CHG context: 5.3% C methylated in CHH context: 4.8% C methylated in unknown context (CN or CHN): 20.0% Bismark completed in 0d 0h 7m 36s