Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth8.fastp-trim.202003060943_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth8.fastp-trim.202003060943_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 11892 Mapping efficiency: 11.9% Sequence pairs with no alignments under any condition: 87173 Sequence pairs did not map uniquely: 935 Sequence pairs which were discarded because genomic sequence could not be extracted: 7 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 2988 ((converted) top strand) GA/CT/CT: 3036 (complementary to (converted) top strand) GA/CT/GA: 3004 (complementary to (converted) bottom strand) CT/GA/GA: 2857 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 541795 Total methylated C's in CpG context: 30803 Total methylated C's in CHG context: 1923 Total methylated C's in CHH context: 7874 Total methylated C's in Unknown context: 480 Total unmethylated C's in CpG context: 46486 Total unmethylated C's in CHG context: 99168 Total unmethylated C's in CHH context: 355541 Total unmethylated C's in Unknown context: 2118 C methylated in CpG context: 39.9% C methylated in CHG context: 1.9% C methylated in CHH context: 2.2% C methylated in unknown context (CN or CHN): 18.5% Bismark completed in 0d 0h 0m 22s