Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth5.fastp-trim.202003064408_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth5.fastp-trim.202003064408_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 51212 Mapping efficiency: 51.2% Sequence pairs with no alignments under any condition: 41868 Sequence pairs did not map uniquely: 6920 Sequence pairs which were discarded because genomic sequence could not be extracted: 31 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 12492 ((converted) top strand) GA/CT/CT: 13353 (complementary to (converted) top strand) GA/CT/GA: 13196 (complementary to (converted) bottom strand) CT/GA/GA: 12140 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 2267932 Total methylated C's in CpG context: 54132 Total methylated C's in CHG context: 5018 Total methylated C's in CHH context: 11580 Total methylated C's in Unknown context: 271 Total unmethylated C's in CpG context: 433217 Total unmethylated C's in CHG context: 439317 Total unmethylated C's in CHH context: 1324668 Total unmethylated C's in Unknown context: 4740 C methylated in CpG context: 11.1% C methylated in CHG context: 1.1% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 5.4% Bismark completed in 0d 0h 0m 35s