Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth3.fastp-trim.202003064031_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth3.fastp-trim.202003064031_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 39540 Mapping efficiency: 39.5% Sequence pairs with no alignments under any condition: 59056 Sequence pairs did not map uniquely: 1404 Sequence pairs which were discarded because genomic sequence could not be extracted: 19 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9337 ((converted) top strand) GA/CT/CT: 10383 (complementary to (converted) top strand) GA/CT/GA: 10474 (complementary to (converted) bottom strand) CT/GA/GA: 9327 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1743548 Total methylated C's in CpG context: 8689 Total methylated C's in CHG context: 3922 Total methylated C's in CHH context: 13892 Total methylated C's in Unknown context: 967 Total unmethylated C's in CpG context: 260749 Total unmethylated C's in CHG context: 315223 Total unmethylated C's in CHH context: 1141073 Total unmethylated C's in Unknown context: 6526 C methylated in CpG context: 3.2% C methylated in CHG context: 1.2% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 12.9% Bismark completed in 0d 0h 1m 4s