Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth5.fastp-trim.202003064408_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth5.fastp-trim.202003064408_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 278504046 Number of paired-end alignments with a unique best hit: 1067419 Mapping efficiency: 0.4% Sequence pairs with no alignments under any condition: 277291564 Sequence pairs did not map uniquely: 145063 Sequence pairs which were discarded because genomic sequence could not be extracted: 484 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 258954 ((converted) top strand) GA/CT/CT: 279269 (complementary to (converted) top strand) GA/CT/GA: 274750 (complementary to (converted) bottom strand) CT/GA/GA: 253962 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 47274700 Total methylated C's in CpG context: 1130561 Total methylated C's in CHG context: 107612 Total methylated C's in CHH context: 247195 Total methylated C's in Unknown context: 4962 Total unmethylated C's in CpG context: 8998972 Total unmethylated C's in CHG context: 9137978 Total unmethylated C's in CHH context: 27652382 Total unmethylated C's in Unknown context: 96514 C methylated in CpG context: 11.2% C methylated in CHG context: 1.2% C methylated in CHH context: 0.9% C methylated in unknown context (CN or CHN): 4.9% Bismark completed in 0d 1h 22m 40s