Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth4.fastp-trim.202003062011_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth4.fastp-trim.202003062011_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 146726267 Number of paired-end alignments with a unique best hit: 40217655 Mapping efficiency: 27.4% Sequence pairs with no alignments under any condition: 101010131 Sequence pairs did not map uniquely: 5498481 Sequence pairs which were discarded because genomic sequence could not be extracted: 20680 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 9915568 ((converted) top strand) GA/CT/CT: 10354638 (complementary to (converted) top strand) GA/CT/GA: 10209566 (complementary to (converted) bottom strand) CT/GA/GA: 9717203 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1749438713 Total methylated C's in CpG context: 43710315 Total methylated C's in CHG context: 4930932 Total methylated C's in CHH context: 12331107 Total methylated C's in Unknown context: 206187 Total unmethylated C's in CpG context: 334058971 Total unmethylated C's in CHG context: 338863365 Total unmethylated C's in CHH context: 1015544023 Total unmethylated C's in Unknown context: 3574152 C methylated in CpG context: 11.6% C methylated in CHG context: 1.4% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 5.5% Bismark completed in 0d 6h 44m 5s