Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth2.fastp-trim.202003062630_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth2.fastp-trim.202003062630_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 83250476 Number of paired-end alignments with a unique best hit: 7456720 Mapping efficiency: 9.0% Sequence pairs with no alignments under any condition: 75525880 Sequence pairs did not map uniquely: 267876 Sequence pairs which were discarded because genomic sequence could not be extracted: 2786 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1746089 ((converted) top strand) GA/CT/CT: 1978861 (complementary to (converted) top strand) GA/CT/GA: 1980469 (complementary to (converted) bottom strand) CT/GA/GA: 1748515 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 320124616 Total methylated C's in CpG context: 1490490 Total methylated C's in CHG context: 732469 Total methylated C's in CHH context: 2668293 Total methylated C's in Unknown context: 166679 Total unmethylated C's in CpG context: 47936200 Total unmethylated C's in CHG context: 58087264 Total unmethylated C's in CHH context: 209209900 Total unmethylated C's in Unknown context: 1132871 C methylated in CpG context: 3.0% C methylated in CHG context: 1.2% C methylated in CHH context: 1.3% C methylated in unknown context (CN or CHN): 12.8% Bismark completed in 0d 1h 35m 39s