Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth1.fastp-trim.202003061245_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth1.fastp-trim.202003061245_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pdam_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 72851576 Number of paired-end alignments with a unique best hit: 29909743 Mapping efficiency: 41.1% Sequence pairs with no alignments under any condition: 41941712 Sequence pairs did not map uniquely: 1000121 Sequence pairs which were discarded because genomic sequence could not be extracted: 15036 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 7000099 ((converted) top strand) GA/CT/CT: 7937526 (complementary to (converted) top strand) GA/CT/GA: 7944576 (complementary to (converted) bottom strand) CT/GA/GA: 7012506 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1314570373 Total methylated C's in CpG context: 6308155 Total methylated C's in CHG context: 2922305 Total methylated C's in CHH context: 10444004 Total methylated C's in Unknown context: 743676 Total unmethylated C's in CpG context: 194808095 Total unmethylated C's in CHG context: 236105269 Total unmethylated C's in CHH context: 863982545 Total unmethylated C's in Unknown context: 4755326 C methylated in CpG context: 3.1% C methylated in CHG context: 1.2% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 13.5% Bismark completed in 0d 5h 23m 5s