Bismark report for: /gscratch/scrubbed/sr320/froger-raw/00_fastq/Meth2_R1_001.fastq.gz and /gscratch/scrubbed/sr320/froger-raw/00_fastq/Meth2_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 1152 Mapping efficiency: 11.5% Sequence pairs with no alignments under any condition: 8815 Sequence pairs did not map uniquely: 33 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 266 ((converted) top strand) GA/CT/CT: 288 (complementary to (converted) top strand) GA/CT/GA: 324 (complementary to (converted) bottom strand) CT/GA/GA: 273 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 66590 Total methylated C's in CpG context: 329 Total methylated C's in CHG context: 141 Total methylated C's in CHH context: 497 Total methylated C's in Unknown context: 21 Total unmethylated C's in CpG context: 9717 Total unmethylated C's in CHG context: 12032 Total unmethylated C's in CHH context: 43874 Total unmethylated C's in Unknown context: 49 C methylated in CpG context: 3.3% C methylated in CHG context: 1.2% C methylated in CHH context: 1.1% C methylated in unknown context (CN or CHN): 30.0% Bismark completed in 0d 0h 2m 42s