Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth6.fastp-trim.202003050737_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth6.fastp-trim.202003050737_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 4339 Mapping efficiency: 43.4% Sequence pairs with no alignments under any condition: 4931 Sequence pairs did not map uniquely: 730 Sequence pairs which were discarded because genomic sequence could not be extracted: 4 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1050 ((converted) top strand) GA/CT/CT: 1095 (complementary to (converted) top strand) GA/CT/GA: 1168 (complementary to (converted) bottom strand) CT/GA/GA: 1022 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 192824 Total methylated C's in CpG context: 5291 Total methylated C's in CHG context: 369 Total methylated C's in CHH context: 823 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 37578 Total unmethylated C's in CHG context: 37698 Total unmethylated C's in CHH context: 111065 Total unmethylated C's in Unknown context: 65 C methylated in CpG context: 12.3% C methylated in CHG context: 1.0% C methylated in CHH context: 0.7% C methylated in unknown context (CN or CHN): 0.0% Bismark completed in 0d 0h 1m 28s