Bismark report for: /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth4.fastp-trim.202003055221_R1_001.fastq.gz and /gscratch/scrubbed/samwhite/outputs/20200305_methcompare_fastp_trimming/Meth4.fastp-trim.202003055221_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000 Number of paired-end alignments with a unique best hit: 4399 Mapping efficiency: 44.0% Sequence pairs with no alignments under any condition: 4810 Sequence pairs did not map uniquely: 791 Sequence pairs which were discarded because genomic sequence could not be extracted: 10 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1076 ((converted) top strand) GA/CT/CT: 1120 (complementary to (converted) top strand) GA/CT/GA: 1116 (complementary to (converted) bottom strand) CT/GA/GA: 1077 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 194583 Total methylated C's in CpG context: 5428 Total methylated C's in CHG context: 406 Total methylated C's in CHH context: 792 Total methylated C's in Unknown context: 4 Total unmethylated C's in CpG context: 38019 Total unmethylated C's in CHG context: 38575 Total unmethylated C's in CHH context: 111363 Total unmethylated C's in Unknown context: 117 C methylated in CpG context: 12.5% C methylated in CHG context: 1.0% C methylated in CHH context: 0.7% C methylated in unknown context (CN or CHN): 3.3% Bismark completed in 0d 0h 2m 32s