Bismark report for: /gscratch/srlab/sr320/data/froger/trim/Pa/Meth1.fastp-trim.202003061245_R1_001.fastq.gz and /gscratch/srlab/sr320/data/froger/trim/Pa/Meth1.fastp-trim.202003061245_R2_001.fastq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Pact_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 72851576 Number of paired-end alignments with a unique best hit: 307226 Mapping efficiency: 0.4% Sequence pairs with no alignments under any condition: 72525273 Sequence pairs did not map uniquely: 19077 Sequence pairs which were discarded because genomic sequence could not be extracted: 1372 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 71554 ((converted) top strand) GA/CT/CT: 80775 (complementary to (converted) top strand) GA/CT/GA: 81579 (complementary to (converted) bottom strand) CT/GA/GA: 71946 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 13571525 Total methylated C's in CpG context: 67929 Total methylated C's in CHG context: 29960 Total methylated C's in CHH context: 105709 Total methylated C's in Unknown context: 8061 Total unmethylated C's in CpG context: 2015111 Total unmethylated C's in CHG context: 2432486 Total unmethylated C's in CHH context: 8920330 Total unmethylated C's in Unknown context: 46818 C methylated in CpG context: 3.3% C methylated in CHG context: 1.2% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 14.7% Bismark completed in 0d 0h 25m 26s